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Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) <t>Co-immunofluorescence</t> staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
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1) Product Images from "RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation"

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

Journal: Molecular Metabolism

doi: 10.1016/j.molmet.2025.102175

Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.
Figure Legend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Techniques Used: Western Blot, Immunofluorescence, Staining

Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.
Figure Legend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Techniques Used: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing

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Fluorescence:

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Software:

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Activation Assay:

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Concentration Assay:

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Transplantation Assay:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
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Gene Expression:

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Expressing:

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Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

DNA Extraction:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Amplification:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Sequencing:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Enzyme-linked Immunosorbent Assay:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Immunofluorescence:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Western Blot:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Staining:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Immunohistochemistry:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Phospho-proteomics:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Immunostaining:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Immunohistochemical staining:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Saline:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Real-time Polymerase Chain Reaction:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Incubation:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Control:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Imaging:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.

Cell Culture:

Article Title: Synthesis and structure-activity relationship of boronic acid bioisosteres of combretastatin A-4 as anticancer agents.
Article Snippet: Cells were examined with a Nikon ES800 fluorescence microscope, and images were captured with a Photometrics Cool Snap FX3 camera and compiled using Metamorph® software.

Article Title: The in vivo blood compatibility of bio-inspired small diameter vascular graft: effect of submicron longitudinally aligned topography
Article Snippet: With the help of software Metamorph, we calculated the adhesion rate of the activated platelets on different vascular grafts using the following equation:M Adhesion rate ‰ = area adhered with activated platelets total field area × 1000 ‰ We observed that there was no significant difference between the adhesion rates on different vascular grafts (smooth/aligned: 0.35‰/0.12‰, p = 0.058), although they had showed an obvious difference in patency rate (Table ).

Article Title: Therapeutic Potential of Microbiota Modulation in Alzheimer’s Disease: A Review of Preclinical Studies
Article Snippet: Following this, confocal microscopy allowed the visualization of results and software were used to quantify Aβ deposits, such as MetaMorph [ ].

Article Title: Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells.
Article Snippet: Glucagon like peptide-1 (GLP-1) is one of incretin hormone and is secreted when enteroendocrine L cells sense saccharides, amino acids, and fatty acids.. Some amino acids have been shown to promote GLP-1 secretion from small intestinal enteroendocrine L cells.. However, the molecular mechanisms that Lphenylalanine, a potent trigger of GLP-1 secretion, causes GLP-1 secretion from enteroendocrine L cells has not been elucidated.

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: QD recording sessions were processed with the software Metamorph.

Article Title: Direct imaging and automatic analysis in tumor-on-chip reveal cooperative antitumoral activity of immune cells and oncolytic vaccinia virus.
Article Snippet: Organ-on-chip and tumor-on-chip microfluidic cell cultures represent a fast-growing research field for modelling organ functions and diseases, for drug development, and for promising applications in personalized medicine.. Still, one of the bottlenecks of this technology is the analysis of the huge amount of bio-images acquired in these dynamic 3D microenvironments, a task that we propose to achieve by exploiting the interdisciplinary contributions of computer science and electronic engineering.. In this work, we apply this strategy to the study of oncolytic vaccinia virus (OVV), an emerging agent in cancer immunotherapy.

Article Title: The ACE2 decoy receptor can overcome immune escape by rapid mutating SARS-CoV-2 variants and reduce cytokine induction and clot formation.
Article Snippet: Fusion events were visualized using a Zeiss Axiovert 200 M microscope, with green fluorescence quantified by MetaMorph software.

Article Title: Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors
Article Snippet: Cells were photographed using a Nikon TE200 fluorescence microscope with the Metamorph software package at indicated magnifications.



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Image Search Results


Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Ablating Pcbp1 in β cells upregulates PCBP2 and does not alter glucose homeostasis. (A) Model of Cre-mediated recombination of Pcbp1 . The top diagram represents the Pcbp1 locus (light boxes represent UTRs, dark box represents protein coding region, and arrow represents transcriptional start site) with inserted loxP sites (triangles). Bottom diagram represents the Pcbp1 locus following RIP-Cre mediated recombination and remaining loxP site (triangle). (B) Western blot showing PCBP1 levels in islets from 26 week old male Pcbp1 βKO mice ( Pcbp1 Fl/Fl ,n = 3; Pcbp1 βKO ,n = 3). (C) Co-immunofluorescence staining for PCBP1 and Insulin in adult islets from 7 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice (scale bar, 50 μm). (D) Intraperitoneal glucose tolerance tests performed on 8–11 week old male mice (n = 9 Pcbp1 Fl/Fl and n = 11 Pcbp1 βKO ). (E) Western blots showing PCBP2 levels in islets from 26 week old male Pcbp1 Fl/Fl and Pcbp1 βKO mice ( Pcbp1 Fl/Fl , n = 3; Pcbp1 βKO , n = 3). ∗P-value<0.05, ∗∗P-value<0.01 by student's t-test.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: Western Blot, Immunofluorescence, Staining

Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Journal: Molecular Metabolism

Article Title: RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translation

doi: 10.1016/j.molmet.2025.102175

Figure Lengend Snippet: Single cell RNA sequencing of Pcbp1 and Pcbp2 co-depleted β cells reveals dysregulation of a translational mRNA program. (A) Co-immunofluorescence staining for PCBP1 and PCBP2 in adult islets from 11–12 week old male Pcbp1/2 Fl/Fl and Pcbp1/2 βKO mice (scale bar, 50 μm). Yellow arrows denote PCBP1 and PCBP2 co-depleted β cells. (B) Merged uniform manifold approximation and projection (UMAP) plot showing 3,592 control β cells (red) and 1,554 Pcbp1/2 co-depleted β cells (blue). (C) UMAP plot showing identified β cell subclusters in control and mutant β cells. (D) Dotplot showing the relative expression of Ins1 , Pcbp1 , and Pcbp2 transcript levels in each identified β cell subcluster. (E) Volcano plot of genes with significantly downregulated (blue) and upregulated (red) transcript levels in cluster 11 as compared to control β cells. (F) Reactome pathway analysis of the genes with altered mRNA levels in the setting of Pcbp1/2 co-deficiency.

Article Snippet: Immunofluorescence images were visualized and captured using a Nikon Eclipse E600 microscope equipped with a QImaging Q/Click digital camera and Metamorph software.

Techniques: RNA Sequencing, Immunofluorescence, Staining, Control, Mutagenesis, Expressing